ANTI-SARS-COV-2 N ANTIBODY CLONE 3C8 or 6E4-B9
Domain Health and wellbeing
Technology New immunotherapy modalities
Description
Anti-SARS-CoV-2 N (nucleoprotein) Antibody, clone 3C8 or 6E4-B9, hybridoma cell line
ALTERNATE NAMES
• Nucleoprotein
• Nucleocapsid protein
• NC
• N
BACKGROUND INFORMATION/DESCRIPTION
Nucleoprotein (UniProt: P0DTC9, also known as nucleocapsid protein, NC or N) is encoded by the N gene in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). The SARS-CoV-2 particle is composed of four polypetides and a genomic RNA. The nucleoprotein N is part of the viral particle binding and protecting the encapsidated RNA genome. The nucleoprotein binds to the envelope E and membrane M capsid proteins and is involved in packaging of the genomic RNA. The nucleoprotein N also suppresses interferon signaling. Nucleoprotein N has been suggested as drug target and as diagnostic target. Clone 3C8 or 6E4-B9 both recognizes SARS-Cov-2-N but not MERS-CoV-N.
Product Information
FORMAT
Hybridoma
PRESENTATION
Hybridoma clone, 1 vial with 10/6 cells
Applications
APPLICATION
Anti-SARS-CoV-2 N (nucleoprotein) Antibody, clone 3C8 or 6E4-B9, are both hybridoma cell lines producing mouse monoclonal antibody that detects the SARS-Cov-2 nucleoprotein N and is tested for use in Western Blotting, Immunofluorescence and ELISA.
KEY APPLICATIONS
Western Blotting, Immunofluorescence
APPLICATION NOTES
Immunofluorescence Analysis: A representative lot of each clone 3C8 or 6E4-B9 detected SARS-CoV-2-N in SARS-CoV-2 infected Vero E6 cells or U2OS cells stably expressing the nucleoprotein.
Western blot analysis: A representative lot detected recombinant SARS-CoV-2-N.
Biological Information
|
Immunogen |
bacterially expressed and purified SARS-CoV-2-N protein |
|
Clone |
clone 3C8 or 6E4-B9 |
|
Concentration |
N.A. |
|
Host |
Mouse |
|
Specificity |
Clone 3C8 or 6E4-B9 are hybridomas producing mouse monoclonal antibody that detects SRS-CoV-2-N protein. |
|
Isotype |
IgG1 kappa |
|
Species Reactivity |
virus |
|
Antibody Type |
monoclonal antibody |
|
NCBI Gene ID |
43740575 |
|
Gene symbol |
N |
|
Purification Method |
Not purified (can be purified on Protein G sepharose to >1mg/ml) |
|
UniProt number |
P0DTC9 (NCAP_SARS2) |
|
Molecular Weight |
45.6 kDa |
Product usage statements
QUALITY ASSURANCE
Evaluated by Western Blotting using recombinant SARS-CoV-2-N protein and Immune fluorescence of infected Vero E6 cells or U2OS cells stably expressing the nucleoprotein. Western Blotting Analysis: 0.4 µg/mL of this antibody detected 100ng of protein by western blot. 2 µg/mL of this antibody detected infected cells by Immunofluorescence analysis.
USAGE STATEMENT
These products can be used for research or authorized commercial uses. This product has not been validated for in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. storage and shipping information
STORAGE CONDITIONS
Hybridoma cells are stable for 6 months at -80°C from date of receipt. packaging information
MATERIAL SIZE
1 vial with 10/6 cells
Contact
Jean-Luc CHAGNAUD
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